A practical guide to scanning light-field microscopy with digital adaptive optics

Zhi Lu, Yeyi Cai, Yixin Nie, Yuxin Yang, Jiamin Wu, Qionghai Dai

Published: 2022-06-28 DOI: 10.1038/s41596-022-00703-9

Scanning Light-Field Microscopy

Digital Adaptive Optics

Intravital Fluorescence Imaging

3D Reconstruction

Open-Source GUI

AI 解读

Generation of mouse–human chimeric embryos

Genome-wide quantification of transcription factor binding at single-DNA-molecule resolution using methyl-transferase footprinting

Generation of CRISPR–Cas9-mediated genetic knockout human intestinal tissue–derived enteroid lines by lentivirus transduction and single-cell cloning

A puromycin-dependent activity-based sensing probe for histochemical staining of hydrogen peroxide in cells and animal tissues

Decoding personal biotic and abiotic airborne exposome

A clinically relevant model of focal embolic cerebral ischemia by thrombus and thrombolysis in rhesus monkeys

Bioengineering human intestinal mucosal grafts using patient-derived organoids, fibroblasts and scaffolds

Biomimetic tail-to-head terpene cyclizations using the resorcin[4]arene capsule catalyst

Neuroscience robotics for controlled induction and real-time assessment of hallucinations

Scanorama: integrating large and diverse single-cell transcriptomic datasets

Single-cell RNA sequencing (scRNA-seq)（单细胞RNA测序）

Data integration（数据集成）

Heterogeneity（异质性）

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With the development of a wide variety of animal models in recent years, there is a rapidly growing demand for long-term, high-speed intravital fluorescence imaging to observe intercellular and intracellular interactions in their native states. Scanning l
Introduction
Materials
- Reagents
- Equipment
Procedure
Troubleshooting
Timing
Anticipated results
References
ACKNOWLEDGMENTS

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A practical guide to scanning light-field microscopy with digital adaptive optics

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