Generation of CRISPR–Cas9-mediated genetic knockout human intestinal tissue–derived enteroid lines by lentivirus transduction and single-cell cloning

Shih-Ching Lin, Kei Haga, Xi-Lei Zeng, Mary K. Estes

Published: 2022-02-22 DOI: 10.1038/s41596-021-00669-0

CRISPR-Cas9

genetic knockout

intestinal organoids

lentivirus transduction

single-cell cloning

AI 解读

Generation of mouse–human chimeric embryos

Genome-wide quantification of transcription factor binding at single-DNA-molecule resolution using methyl-transferase footprinting

Generation of CRISPR–Cas9-mediated genetic knockout human intestinal tissue–derived enteroid lines by lentivirus transduction and single-cell cloning

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Decoding personal biotic and abiotic airborne exposome

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Bioengineering human intestinal mucosal grafts using patient-derived organoids, fibroblasts and scaffolds

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Single-cell RNA sequencing (scRNA-seq)（单细胞RNA测序）

Data integration（数据集成）

Heterogeneity（异质性）

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Human intestinal tissue–nobreakderived enteroids (HIEs; also called organoids) are a powerful exvivo model for gastrointestinal research. Genetic modification of these nontransformed cultures allows new insights into gene function and biological
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ACKNOWLEDGMENTS

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Generation of CRISPR–Cas9-mediated genetic knockout human intestinal tissue–derived enteroid lines by lentivirus transduction and single-cell cloning

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