Automated human induced pluripotent stem cell culture and sample preparation for 3D live-cell microscopy

Nathalie Gaudreault, Benjamin W. Gregor, Mackenzie E. Coston, Ellen M. Adams, Joy Arakaki, Antoine Borensztejn, Thao P. Do, Margaret A. Fuqua, Amanda Haupt, Melissa C. Hendershott, Winnie Leung, Irina A. Mueller, Aditya Nath, Angelique M. Nelson, Susanne M. Rafelski, Emmanuel E. Sanchez, Madison J. Swain-Bowden, W. Joyce Tang, Derek J. Thirstrup, Winfried Wiegraebe, Brian P. Whitney, Calysta Yan, Ruwanthi N. Gunawardane

Published: 2023-12-12 DOI: 10.1038/s41596-023-00912-w

deep learning

automation

hiPS cells

3D microscopy

image datasets

AI 解读

Generation of mouse–human chimeric embryos

Genome-wide quantification of transcription factor binding at single-DNA-molecule resolution using methyl-transferase footprinting

Generation of CRISPR–Cas9-mediated genetic knockout human intestinal tissue–derived enteroid lines by lentivirus transduction and single-cell cloning

A puromycin-dependent activity-based sensing probe for histochemical staining of hydrogen peroxide in cells and animal tissues

Decoding personal biotic and abiotic airborne exposome

A clinically relevant model of focal embolic cerebral ischemia by thrombus and thrombolysis in rhesus monkeys

Bioengineering human intestinal mucosal grafts using patient-derived organoids, fibroblasts and scaffolds

Biomimetic tail-to-head terpene cyclizations using the resorcin[4]arene capsule catalyst

Neuroscience robotics for controlled induction and real-time assessment of hallucinations

Scanorama: integrating large and diverse single-cell transcriptomic datasets

Single-cell RNA sequencing (scRNA-seq)（单细胞RNA测序）

Data integration（数据集成）

Heterogeneity（异质性）

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References

Introduction
Materials
Procedure 1: automated Matrigel coating (workflow 3)
Procedure 2: manual cell thawing and seeding
Procedure 3: automated plate maintenance (workflow 1)
Procedure 4: automated cell passaging (workflow 2)
Troubleshooting
Timing
Anticipated results
Acknowledgements

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Automated human induced pluripotent stem cell culture and sample preparation for 3D live-cell microscopy

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