Multiplexed single-cell proteomics using SCoPE2

Andrew Leduc, Nikolai Slavov, Aleksandra A. Petelski, Edward Emmott, R. Gray Huffman, Harrison Specht, David H. Perlman

Published: 2021-10-28 DOI: 10.1038/s41596-021-00616-z

Extended

Extended Data Fig. 1 The ABIRD may suppress contaminant ions and enhance peptide identification.

ABIRD may suppress contaminant ions and enhance peptide identification. Replicate injections of a 1× standard were analyzed with the ABIRD on or off. a , The replicates with ABIRD on had a reduced number of +1 ions (likely corresponding to contaminants) and an increased number of higher-charge-state ions, which are likely to correspond to peptides. b , With the ABIRD on, the number of identified peptides is increased across all confidence levels (PEP).

Source data

Supplementary information

Supplementary Data 1

XML file for MaxQuant that specifies the parameters for TMTpro 16-plex searches.

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Multiplexed single-cell proteomics using SCoPE2

Extended

Extended Data Fig. 1 The ABIRD may suppress contaminant ions and enhance peptide identification.

Supplementary information

Supplementary Data 1

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