SAN:

SPEC No:

OPERATOR & STEPS:

OPERATOR & STEPS:

Using diamond pen etch a circle on the back of the slide to indicate where the BEEM capsule should be placed during embedding.

Remove coverslip by soaking in minimum , check to see if coverslip has slide off, if not soak another .

100% volume 0h 5m 0s

95% volume 0h 5m 0s

70% volume 0h 5m 0s

2.5% volume in 0.1Molarity (M) Sorenson's Phosphate buffer (pH 7.2, 300mosmol/kg) for at least 0h 5m 0s

0.1Molarity (M) buffer 0h 5m 0s

1% volume in buffer 0h 30m 0s

70% volume for at least 0h 5m 0s or overnight

95% volume 0h 5m 0s

100% volume 0h 5m 0s

100% volume dried with molecular sieve 0h 5m 0s

50% volume 50% volume 0h 10m 0s

100% volume 0h 10m 0s

50% volume 50% volume 0h 20m 0s

100% volume 0h 20m 0s

100% volume 1h 0m 0s

Embed & polymerise overnight in oven at 60°C

Place slide into boiling water on hot plate setting 3 for 0h 9m 0s .Remove from water, gloves on, use tissues to hold slide down, angle single edged razor blade at 45o underneath tap washer to make a start to removal , then manually break BEEM capsule off slide.